Nuclear Fusion and Genome Encounter during Yeast Zygote Formation
نویسندگان
چکیده
منابع مشابه
Nuclear fusion and genome encounter during yeast zygote formation.
When haploid cells of Saccharomyces cerevisiae are crossed, parental nuclei congress and fuse with each other. To investigate underlying mechanisms, we have developed assays that evaluate the impact of drugs and mutations. Nuclear congression is inhibited by drugs that perturb the actin and tubulin cytoskeletons. Nuclear envelope (NE) fusion consists of at least five steps in which preliminary ...
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Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation
Zygote formation occurs through tightly coordinated cell and nuclear fusion events. Genetic evidence suggests that the FUS2 gene product promotes cell fusion during zygote formation in Saccharomyces cerevisiae, functioning with the Fus1 plasma membrane protein at or before cell wall and plasma membrane fusion. Here we report the sequence of the FUS2 gene, localization of Fus2 protein, and show ...
متن کاملDistinct roles for key karyogamy proteins during yeast nuclear fusion.
During yeast mating, cell fusion is followed by the congression and fusion of the two nuclei. Proteins required for nuclear fusion are found at the surface (Prm3p) and within the lumen (Kar2p, Kar5p, and Kar8p) of the nuclear envelope (NE). Electron tomography (ET) of zygotes revealed that mutations in these proteins block nuclear fusion with different morphologies, suggesting that they act in ...
متن کاملEvidence for thylakoid membrane fusion during zygote formation in Chlamydomonas reinhardtii
To understand whether fusions of thylakoid membranes from the parental chloroplasts occurred during zygote formation in Chlamydomonas reinhardtii, we performed an ultrastructural analysis of the zygotes produced by crossing mutants lacking photosystem I or II protein complexes, in the absence of de novo chloroplast protein synthesis. Thylakoid membranes from each parent could be distinguished o...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2009
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e08-12-1193